【摘要】 为了研究从普通级净化到 SPF级前后的 KM小鼠免疫功能变化的特点 ,对同一封闭群的KM小鼠在净化前后分别进行免疫功能的测定。结果 KM小鼠净化前后抗体生成细胞分别是5 5 7.39± 85 .77和 368.62± 65 .2 3;血清抗体水平分别是 67.8± 2 6.0 8和 46.4± 1 5 .45、碳廓清指数 K分别是 5 .2 4± 0 .96和 4.61± 0 .61 ;碳廓清吞噬指数 α分别是 0 .0 32± 0 .0 2 3和 0 .0 2 5± 0 .0 1 ;小鼠巨噬细胞吞噬鸡红细胞的指数分别为 0 .41± 0 .0 6和 0 .36± 0 .0 6,吞噬百分数分别为 38.42± 6.1 5和31 .5 5± 5 .75 ;Con A诱导小鼠脾淋巴细胞转化能力 OD值分别为 0 .0 32± 0 .0 2和 0 .0 31± 0 .0 1 ;二硝基氟苯诱导小鼠迟发型变态反应 (耳肿胀法 )小鼠耳重差分别为 1 0 .85± 4.34mg和 1 6.2 9± 4.2 1mg。结果表明 ,净化后的 KM小鼠的巨噬细胞活力、B淋巴细胞活性、抗体水平均小于净化前 ,有极显著的差异 ( P<0 .0 1 ) ;二硝基氟苯诱导小鼠迟发型变态反应 ( DHT)大于净化前 ,有极显著的差异 ( P<0 .0 1 ) ;Con A诱导小鼠脾淋巴细胞转化能力没有变化。更多还原

【Abstract】 In order to observe the changes of immune function in KM mice before and after biocleaning, immunological examinations were conducted. The function of antibody formation cells was 557.39±85.7 and 368.62±65.23, the antibody level in serum was 67.8±26.08 and 46.4±15.45, the function of removing carbonaceous granule index K was 5.24±0.96 and 4.61±0.61, α was 0.0032±0.0023 and 0.0025±0.001, the phagocytic function of macrophages in mice’s abdominal cavities 0.41±0.66 and 0.36±0.06, the mice’s lymphocyte transformation induced by ConA (OD value) 0.023±0.02 and 0.031±0.001,the mice’s DTH induced by DNFB 10.85±4.34 and 16.29±4.21 before and after biocleaning respectively. It was suggested that some of examined immune function changed significantly after biocleaning such as the phagocytic activity, B lymphatic cells activity and antibody levels. However, no changes were observed in mice’s lymphocyte transformation induced by ConA.更多还原