【摘要】 SARS Cov是引起非典型性肺炎的病原体。S和M蛋白都是SARS冠状病毒主要的膜结构蛋白。研究显示 ,冠状病毒感染细胞过程中 ,病毒表面膜结合蛋白起决定性作用。通过网络数据库结合生物软件分析的方法预测可能在SARS Cov感染过程中起重要作用并具较好抗原性参数的膜结合蛋白结构区域作为抗原表位。构建多表位串联基因片段 ,接入载体pET 32a后验证表明 ,该多表位串联基因可以在原核细胞内高效表达 ,从而为基于表位的SARS Cov疫苗的进一步研制奠定了基础更多还原

【Abstract】 Genomic data showed that the S and M p rotein were those structural proteins critical for the initiation of the infections induced by SARS-Cov. Earlier investi gations had demonstrated that the specific anti-S or anti-M antibody could neutralize the activities of this virus in vitro, with or without the actions of complement system. In the present study, through the combination of BLAST wi th other public software, several candidate epitopes that might play an importan t role in the course of SARS-Cov infection and have better antigenicity were predicted, and the gene encoding those tandem-arranged epitopes were then optimized. After cloned into vector pET-32a, the gene fragment was proved to be able to express in E.coli efficiently. It suggests that this multi-epitope gene is a suita ble candidate for the development of the epitope-based SARS-Cov vaccine.更多还原