【摘要】 目的:探讨口腔癌患者手术前后免疫功能状态变化及其临床意义。方法:采集口腔癌患者手术前后的外周静脉血,流式细胞仪检测CD3+总T淋巴细胞、CD4+辅助性T细胞、CD8+杀伤/抑制性T细胞、CD4+/CD8+比值、CD19+总B淋巴细胞、CD16+/CD56+NK细胞。酶联免疫吸附法(ELISA)检测外周血肿瘤坏死因子-α(TNF-α)和可溶性白细胞介素-2受体(sIL-2R)的表达。采用配对t检验进行统计学分析。结果:口腔癌术后外周静脉血CD8+杀伤/抑制性T细胞显著减少,CD4+/CD8+细胞比值显著升高,CD19+总B淋巴细胞明显升高,CD16+/CD56+NK细胞显著减少,TNF-α的表达明显减少,其余变化无显著性差异。结论:手术虽然影响口腔癌患者的免疫功能,但减轻肿瘤患者的免疫抑制状态。术后辅助治疗尤其是免疫治疗,对口腔癌患者具有重要意义。更多还原

【Abstract】 PURPOSE:To investigate the immunological function for patients with oral cancer undergoing operation. METHODS:Peripheral blood was taken from patients with oral cancer before and after operation. Proportions of CD3+ total T, CD4+ Th, CD8+ Tc/Ts, CD19+ total B, CD16+/CD56+NK were detected by flow cytometry ( FCM). Detections of serum tumor necrosis factor alpha (TNF-α) and soluble interleukin-2 recepter (sIL-2R) were performed with enzyme-linked immunosorbent assay(ELISA). Paired t test was used for statistical analysis.RESULTS:The proportions of CD8+Tc/Ts, CD19+ total B and CD4+/CD8+ increased significantly after surgery. Post operative proportion of CD16+/CD56+NK decreased more significantly. The concentration of TNF-αwas less than that before operation(P<0.05). CONCLUSIONS:Surgery could change immunological function and alleviate immunosuppression of oral cancer patients. Detections for immune conditions of oral cancer patients might have important clinical values in prognosis evaluation and guiding treatment, especially immunotherapy, after operation.更多还原