【摘要】 目的 :探讨白血病抑制因子 (LIF)基因在子宫蜕膜及绒毛组织中的表达与反复自然流产 (RSA)的关系。方法 :采用逆转录 -聚合酶链反应 (RT -PCR)技术 ,对 2 0例RSA患者和 30例正常妊娠妇女子宫蜕膜和绒毛组织进行LIFmRNA检测。结果 :RSA组蜕膜组织中LIFmRNA的表达水平为 0 5 12± 0 32 6 ( x±s,下同 ) ,正常妊娠组为 1 345± 0 885 ,RSA患者蜕膜组织中LIFmRNA表达显著性降低 (P <0 0 5 ) ;RSA组绒毛组织中LIFmRNA的表达水平为 0 6 0 1± 0 10 5 ,正常妊娠组为 1 75 9± 0 318,前者较后者明显降低 (P <0 0 1)。结论 :LIF对妊娠的维持可能起一定的作用。LIF基因表达减弱可能是导致复发性自然流产的原因之一更多还原

【Abstract】 Objective: To investigate the expression of leukemia inhibitory factor (LIF) gene in the decidual and chorionic tissue of patients with recurrent spontaneous abortions. Methods: By a quantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of LIF gene in the decidual and chorionic tissue was detected in 20 cases of recurrent spontaneous abortion (RSA group) and in 30 normal pregnancy cases (control group). Results: The level of LIFmRNA expression in the decidual and chorionic tissue in RSA group were 0.512±0.326 and 0.601±0.105, respectively, which was significantly lower than that in control group (1.345±0.885 and 1.759±0.318, P<0.05 and P<0.01). Conclusions: Our finding suggested LIF might play an important role in the maintaining of normal pregnancy. The decreased expression of LIF gene might be one of the major reason causing recurrent spontaneous abortions.更多还原