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胰高血糖素样肽-1对胰岛β细胞功能的影响
The effect of glucagon like peptide-1 on pancreatic islet beta cell function
【摘要】 目的:探讨胰高血糖素样肽(GLP)-1对胰岛β细胞功能的影响?方法:Wistar雄性大鼠20只,随机分为对照组(生理盐水)和实验组[GLP-1 (7-36) NH2],每组各10只?分别在注射生理盐水?GLP-1 (7-36)NH2前及结束后5?10 min时测尾部血糖?胰岛素?应用半定量逆转录聚合酶链式反应(RT-PCR)检测胰岛素mRNA表达强度?结果:注射前,对照组与实验组血糖?血胰岛素浓度差异均无统计学意义(P>0.05);注射后5?10 min时实验组血糖显著低于对照组(P<0.05);5 min时实验组胰岛素水平显著高于对照组(P<0.05);10 min时对照组与实验组血胰岛素水平差异无统计学意义(P>0.05)?胰岛素mRNA表达强度实验组显著高于对照组(P<0.05)?结论:静脉注射GLP-1 (7-36) NH2能促进胰岛素基因转录,升高胰腺胰岛素mRNA水平,促进胰岛素的合成与分泌,迅速升高血胰岛素水平,降低血糖,从而保护胰岛β细胞功能?
【Abstract】 Objective: To studay the effects of Glucagon-like peptide-1 on pancreatic islet beta cell function. Methods: Twenty male wistar rats were randomly divided into control group (normal sodium) and experimental group [GLP-1 (7-36) NH2], each group containing ten rats. Tail blood glucose and insulin were assayed before infusion of normal sodium or GLP-1 (7-36) NH2 and at 5 minute, 10 minute after infusion. Insulin mRNA expression intensity was assessed by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Blood glucose and insulin were no significant difference before intravenation between the control and the experiment groups (P>0.05); Concentrations of blood glucose at 5 minute and 10 minute after intravenation were significantly decreased; while concentration of insulin at 5 minute was significantly increased in the experiment group compared with the control one (P<0.05); There was no significant difference at 10 minute in the level of insulin between the control and the experiment (P>0.05). The level of insulin mRNA was significantly higher in the experiment than that of the control(P<0.05). Conclusion: Intravenous injection of GLP-1 (7-36)NH2 can promote insulin gene transcription, increase pancrea’s insulin mRNA levels, stimulate insulin biosynthesis and secretion, increase the level of insulin, decrease blood glucose and protect the function of pancreatic islet beta cell. [
【Key words】 Glucagon-like peptide-1; Insulin; Blood glucose; Islets of langerhans; Polymerase chain reaction;
- 【文献出处】 山东大学学报(医学版) ,Journal of Shandong Univenity(Health Scicnes) , 编辑部邮箱 ,2004年03期
- 【分类号】Q53
- 【被引频次】16
- 【下载频次】350