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NCS对烟草和小麦愈伤组织中细胞内结构的影响

Effects of Narciclsine on Cells of Callis of Wheat and Tobacco

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【作者】 万益琴毕玉蓉刘志学

【Author】 WAN Yi-qin~1,BI Yu-rong~2,LIU Zhi-xue~1 (1.School of Life Sciences, Shanghai University, Shanghai 200436, China; 2.School of Life Sciences, Lanzhou University, Lanzhou 730000, China)

【机构】 上海大学生命科学学院兰州大学生命科学学院上海大学生命科学学院 上海200436兰州730000上海200436

【摘要】 用从多花水仙鳞茎分泌物中分离出的一种生物碱Narciclasine(NCS)处理不同植物的愈伤组织可使其细胞核发生明显的形态变化.可使烟草和小麦的核液泡变小甚至消失、核物质浓缩、核质比减小.在NCS处理的细胞中均观察到了无丝分裂的发生,提示NCS对于细胞的作用方式可能与秋水仙素有所不同.

【Abstract】 Narciclasine is a kind of Amaryllidaceae alkaloid, which is isolated from bulbs of Narcissus tazetta L. The callis of tobacco and wheat were incubated in 10-6 g/L or 10-4 g/L for 1~3 days. (Obvious) changes in the neucleus were observed. For example, volume of vacuole was decreased, the (nuclear)-cytoplasmic ratio lessened, and nuclear matrix condensed. Sometimes amitsis was observed. Such strong inhibitory effect cannot be obtained in the treatment with colchicines of groovy concentration (10 g/L, W/V). The results obtained in our experiment and reported by other researchers indicate that NCS may be used not only as a new plant growth regulator but also as a new anti-cancer medicine.

【关键词】 Narciclasine愈伤组织细胞核
【Key words】 narciclasinecallinucleolous
【基金】 国家自然科学基金资助项目 (3 0 1 70 0 89)
  • 【文献出处】 上海大学学报(自然科学版) ,Journal of Shanghai University(Natural Science Edition) , 编辑部邮箱 ,2004年05期
  • 【分类号】S311
  • 【被引频次】2
  • 【下载频次】69
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