【摘要】 为了研究不同浓度尼古丁对大鼠卵巢黄体细胞存活情况、在有无hCG支持下分泌孕酮、雌二醇的影响 ,将妊娠第 4天的母鼠卵巢中取出的黄体细胞培养于含 5 %胎牛血清的M199培养基中 ,放置于 37℃、5 %CO2 、饱和湿度的培养箱中培养 .大鼠黄体细胞培养 1～ 2小时后 ,分组加入尼古丁生理盐水溶液 ,最终浓度为 :4mg mL、0 4mg mL、0 0 4mg mL、0 0 0 4mg mL、0 0 0 0 4mg/mL ,将大鼠黄体细胞分为两组 ,一组为基础分泌组 ,另一组为hCG刺檄组 .在大鼠黄体细胞受尼古丁作用 4小时后 ,吸取培养液上清部分以放射性免疫法测定孕酮和雌二醇的含量 ,同时用MTT比色法测定黄体细胞存活情况 .结果发现 ,大鼠黄体细胞在有无hCG的刺激下 ,孕酮 ,雌二醇的分泌量均是随着加药浓度的增加而增加 ,大致呈线形关系 ,但其基础分泌量远远小于hCG刺激下的激素分泌量 .更多还原

【Abstract】 The objective of this study was to investigate the effect of nicotin on hormone secretion and cell-survival rat luteal cells.Post-gestational d 4 rat luteal cells were collected and cultured in M1999 medium supplemented with 5% FBS at 37℃ in a humidified atmoshere of 5% CO-2 in air.After 1～2 hours,5 concentrations (4 to 0 0004mg/ml)of nicotin in the presence or absence of hCG was added into the media respectively.The supernet fluids were collected at 4 hours after incubation for measuring estradiol,and progesterone by radioimmunoassay.And the cel survival was evaluated by MTT assay.The results shows that effects of middle concentrations of nicotin were significantly increase the secretion and cell-survival.更多还原