【摘要】 探讨了用大孔吸附树脂从喜树果中提取、纯化喜树碱的工艺。结果表明,以70%乙醇为提取液,浓缩后用饱和石灰水提取,提取液上AB-8树脂,洗脱条件经实验确定为用pH=2,浓度为90%的乙醇洗脱最佳。结晶溶剂为乙酸乙酯/石油醚(2∶3),所得喜树碱纯度可达98%,得率为0 41‰。更多还原

【Abstract】 The optimal method for separation and purification of camptothecin with macroreticular resin from the fruits of Camptotheca acuminata was reported. The results showed that camptothecin was extracted from the fruits of Camptotheca acuminata with 70% alcohol, the extracting solvent was concentrated, the liquid was extracted with saturated limewater and the camptothecin was adsorbed by AB-8 resin. The camptothecin in resin was eluted with 90% alcohol in pH=2 and the crystallizing agent is acetic ester/petroleum ether(2 ∶3). The content of camptothecin in the product was normally 98% and the productivity was 0.41‰.更多还原