【摘要】 通过对国庆1号温州蜜柑GuoqingNo.1(Citruswnshiu)原生质体培养获得的愈伤组织,经体细胞胚发生途径再生植株的研究。结果表明,两步法建立的国庆1号胚性愈伤组织悬浮系,分离的原生质体产量高、活力强,在MA培养基上培养20d时,可获得颗粒性强,具有体细胞胚发生能力的细胞团。这些细胞团在甘油(MT+质量分数2%甘油)培养基上可以获得最好的胚状体诱导效果,但诱导出的小胚状体不能进一步发育,需及时转入液体乳糖(MT+质量分数5%乳糖)培养基上振荡培养20～30d。然后将长至1 0～2 0cm的胚状体转入固体乳糖培养基上,可促使其进一步发育成墨绿色的正常胚状体,最终在生芽培养基上诱导其再生植株。更多还原

【Abstract】 The embryo induction and plantlet regeneration from the Guoqing No.1(Citrus unshiu)callus obtained by protoplast culture were reported in the paper. The results showed that protoplasts isolated from the embryogenic suspension established by two stages demonstrated high productivity and vitality. Embryogenic callus can be obtained after 20d incubation in MA media. Embryo is well-induced in glycerol medium (MT+2% glycerin), followed by culture in liquid lactose medium (MT+5% lactose) for 20-30d and the embryo can developed normally to 1.0-2.0 cm in diameter. To get further development, it should be transferred into solid lactose medium. Plantlets can be regenerated from the shoot induction medium.更多还原