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转生长激素基因彩鲫(Colorful Curcaincarp)的获得及检测

The Construction and Detecting of Transgenic Colorful Curcian carp with Growth Hormone Gene

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【作者】 伍翠芳顾继锐刘会明张文露洪芳蒋天明吴江徐恒

【Author】 WU Cui-fang~1,GU Ji-rui~1,LIU Hui-ming~2,ZHANG Wen-lu~2, HONG Fang~2,WU Jiang~1,JIANG Tian-ming~1,XU Heng~2 (1.Chengdu Tongwei Aquatic Science and Technology Co.Ltd, Chengdu 610081,China; 2.College of Life Science, Sichuan University, Chengdu 610064, China)

【机构】 成都通威水产科技有限公司四川大学生命科学学院四川大学生命科学学院 成都610081成都610081成都610064成都610064

【Abstract】 The recombinant plasmid pFV2cfGH was constructed using Growth hormone gene which was cloned from the channel catfish cDNA library,the β-action promoter of carp and the polyadenylation signal from the salmon growth hormone gene, and recombinant plasmid pFV2cfGH was microinjected into the fertilized eggs of the colorful crucian carp. 60 transgenic Colorful Crucian carps total DNA were got ,and growth hormone gene were detected by PCR. The result shows that the channel catfish growth hormone gene had been transmitted into the genome of the Colorful Crucian carp and the integration rate is about 47%.

【基金】 国家水利部"948"项目;通威集团科技发展项目
  • 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University (Natural Science Edition) , 编辑部邮箱 ,2004年05期
  • 【分类号】Q953
  • 【被引频次】7
  • 【下载频次】82
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