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植物源病毒抑制物WCT-Ⅱ防治烟草花叶病毒的机理初探

Mechanism of Control of Tobacco Mosaic Virus by Antivirus Agent WCT-Ⅱ from Plants

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【摘要】 植物源病毒抑制物WCT-Ⅱ喷施于接种烟草花叶病毒(TMV)的烟草上,与仅接种TMV未经WCT-Ⅱ处理的发病对照相比,烟草叶片中叶绿素含量提高了58.0%,碱溶性胞内几丁质酶和碱溶性胞间几丁质酶活性分别提高27.0% 和40.7%,碱溶性胞内和酸溶性胞间β-1,3-葡聚糖酶活性分别提高22.1% 和140.0%。上述研究结果表明,WCT-Ⅱ能启动植物的一系列抗病防御反应,诱导植物产生抗病性。

【Abstract】 Antivirus agent WCT-II obtained from plants was sprayed to the leaves of tobacco infected with tobacco mosaic virus (TMV), and the infected tobacco without spraying of antivirus agent WCT-II was taken as control. The activities of chitinase and β-1,3-glucanase and the chlorophyll content were determined. The leaf chlorophyll content was found to increase by 58.0% as compared to the control. The activities of basic-soluble chitinase stored in the vacuole and intercellular chitinase increased by 27.0% and 40.7% respectively, and the activities of basic-soluble stored in the vacuole and acid-soluble intercellular β-1,3-glucanase increased by 22.1% and 140.0% respectively in the leaf of tobacco infected by TMV and sprayed by the antivirus agent WCT-II. The results suggested that WCT-II stimulate defense reaction of plant and be induced to produce resistance to pathogens.

【关键词】 WCT-Ⅱ; 叶绿素; 几丁质酶; β-1,3-葡聚糖酶;
【Key words】 WCT-Ⅱ; chlorophyll; chitinase; β-1,3-glucanase;
【基金】 陕西省自然科学基金项目。
  • 【文献出处】 热带农业科学 ,Chinese Journal of Tropical Agriculture , 编辑部邮箱 ,2004年05期
  • 【分类号】S435.72
  • 【被引频次】6
  • 【下载频次】151
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