【摘要】 目的 :研究hTERT基因在HBsAg阳性与阴性肝细胞癌 (hepatocellularcarcinoma ,HCC)患者组织中表达的差异 ,探讨HBV病毒感染与hTERT基因表达在HCC中的相关性。方法 :应用免疫组化 (SP法 )和半定量逆转录聚合酶链反应 (RT PCR)分别检测 73例HCC患者组织中hTERT蛋白及其mRNA的表达情况 ,其中HBsAg阳性 5 3例 ,HBsAg阴性 2 0例 ,比较二者表达的差异。结果 :hTERT蛋白在HBsAg阳性HCC组织中的阳性表达为 48/5 3 ,在HBsAg阴性HCC组织中阳性表达为 12 /2 0 ,两组病例hTERT蛋白表达阳性率差异有统计学意义 ,P <0 0 1;hTERTmRNA在HB sAg阳性HCC组织中阳性表达为 46/5 3 ,hTERTmR NA在HBsAg阴性HCC组织中阳性表达为 11/2 0 ,两组病例hTERTmRNA表达阳性率差异有统计学意义 ,P <0 0 5 ;统计学分析显示 ,HCC中HBsAg与hTERTmRNA的表达有显著关联性 ,与hTERT蛋白的表达强度成系统性的线性趋势 ,P <0 0 1。结论 :HBsAg阳性HCC组织中hTERT基因表达的阳性率和阳性强度均明显高于HBsAg阴性HCC组织 ,差异有统计学意义 ,P <0 0 5 ;HCC中HBV病毒感染与hTERT基因表达之间具有相关性 ,提示在HCC发生发展中可能存在二者的相互作用更多还原

【Abstract】 OBJECTIVE:To investigate the difference in expression of hTERT gene between HBsAg positive human hepatocellular carcinoma (HCC) and HBsAg negative HCC and to explore the relationship between HBV infection and hTERT gene expression in HCC. METHODS: Expression of hTERT protein in HBsAg positive HCC from 53 cases and HBsAg negative HCC from 20 cases was detected by immunohistochemistry (SP method), and hTERT mRNA expression was analyzed by reverse transcription polymerase chain reaction (RT-PCR).T-test,Chi-square test and cochran-armitage trend test were used to estimate whether there was an interrelation between HBsAg and hTERT gene in HCC. RESULTS: Expression of hTERT protein was mostly located in liver cancer cell plasms,occasionally located in nucleus. The positive rates of hTERT protein and hTERT mRNA in HBsAg positive HCC were respectively 48/53 and 46/53 which were much higher than those in the HBsAg negative HCC,i.e. 12/20,11/20,respectively,P<0.05. CONCLUSIONS: HBsAg is related to hTERT gene expression in human HCC. hTERT gene activated by efficacious ingredient of HBV may play an important role in hepatocellular transformation and carcinogenesis.更多还原