【摘要】 对西京医院进行根治性放疗的食管鳞状细胞癌患者 64例 ,照射剂量DT64～ 70Gy ,进行前瞻性研究 ,对其治疗前活检标本进行SP免疫组化染色 ,计数其阳性细胞数 ,并计算阳性率 ,观察其阳性率与食管癌放疗疗效及预后的关系。结果PCNA阳性率 5 1%～ 67 1% ,中央值 3 2 0 % ,PCNA阳性率 >40 %者较低于此值者预后明显差。p16在低分化癌中较高分化癌阳性表达率低 ,且随着病期的进展阳性表达率降低 ,P <0 0 5 ;Ki 67与近期疗效及预后未见明显差异。初步研究结果提示 ,PCNA阳性率是判断放疗疗效有意义的指标 ,且与p16联合检测对食管鳞癌放疗前恶性程度评定和预后估计有重要意义更多还原

【Abstract】 This study is aimed at detecting expressions of PCNA and p16 in esophegeal cancer, and analysing the relationship between their expressions and radiotherapy and prognosis. Totally 64 patients with esophegeal squamouse cell cancer entreed radical radiotherapy study in Xijing Hospital, Fourth Military Medical University. Expressions of PCNA and P16 in patients’ tumour samples were detected by the immunohistochemist method SP before radiotherapy. Then radiotherapy was administered with a radiation dose 64 to 70 Gy in 6 to 7 weeks. Results: The positive rates of PCNA rangeed from 5.1% to 67.1%(median rate was 32.0%). The immediately response rate of radiotherapy has a significant difference between group PCNA positive rate over 40% and group under 40%.The expression of p16 was lower in patients with low differentiation cancer than patients with high differentiation cancer. Accompanied with the development of clinical stage, the positive rate of P16 decreased. However, the expression of ki-67 had no relationship with radiotherapy and prognosis. In conclusion, the positive rate of PCNA is useful to assess the effect of radiotherapy, and its detection with P16 together is important to assess prognosis and malignant degree of esophegeal squamouse cell cancer before radiotherapy.更多还原