【摘要】 目的 :观察全反式维甲酸 (all trans retinoicacid ,ATRA)体外诱导甲状腺癌细胞株的形态学变化和细胞凋亡。方法 :分别以终浓度为 0、10 -7、10 -6、10 -5、10 -4mol/L作用 3种甲状腺癌细胞株(FTC 13 3、K1、85 0 5C) ,在倒置显微镜下观察细胞生长情况及形态特征变化 ;利用Annexin VFITC/PI染色 ,荧光显微镜下观察细胞的凋亡。结果 :ATRA作用后 ,FTC 13 3细胞与对照组比较 ,细胞排列较规整 ,呈梭形 ;对照组K1细胞成团生长 ,经维甲酸作用后细胞成团减少 ;85 0 5C细胞经ATRA处理后 ,形态未见明显变化 ,但ATRA浓度为 10 -4mol/L时可见细胞贴壁能力下降。ATRA在 10 -5、10 -4mol/L时 ,FTC 13 3细胞凋亡率分别为 ( 7 8± 2 6) %和( 11 6± 3 7) % ,K1细胞凋亡率分别为 ( 7 9±2 6) %和 ( 10 8± 3 3 ) %、ATRA在 10 -4mol/L时 ,85 0 5C细胞凋亡率为 ( 15 3± 4 7) % ,与对照组[( 2 8± 0 7) %、( 3 1± 0 9) %、( 6 7± 2 2 ) % ]比较 ,差异均有统计学意义 ,P <0 0 5。结论 :不同浓度的ATRA可诱导FTC 13 3、K1、85 0 5C细胞形态学变化和细胞凋亡。更多还原

【Abstract】 OBJECTIVE:To observe the impact of all-trans-retinoic acid(ATRA) on cell morphology and apoptosis of thyroid cancer cell lines (FTC-133, K1, 8505C) in vitro. METHODS: Treated with different dose of ATRA(final concentration of 0,10 -7 ,10 -6 ,10 -5 and 10 -4 mol/L), the cells were observed with a convert-microscope in shape and with a fluorescence-microscope in apoptosis dyed by Annexin-V FITC/P.RESULTS: Compared to the controls, the cells of FTC-133 grew shuttle-likely and arranged more regularly and gathering of K1 cells decreased. No shape change was found in 8505C cell line, but there was a reduction in the ability of adhering to wall at 10 -4 mol/L ATRA. Apoptosis rates were (7.8±2.6)% and (11.6±3.7)% at high dose of ATRA (10 -5 , 10 -4 mol/L) in FTC-133 , and (7.9±2.6)% and (10.8±3.3)% in K1, respectively. It was (15.3±4.7)% at 10 -4 mol/L of ATRA in 8505C. It was significantly higher compared with that of the control group[(2.8±0.7)%,(3.1±0.9)%,(6.7±2.2)%, P<0.05].CONCLUSION: ATRA can induce morphological changes and apoptosis in these three thyroid cancer cell lines.更多还原