【摘要】 目的　观察尾加压素Ⅱ (UrotensinⅡ ,UⅡ )及其受体UT在慢性低O2 高CO2 肺动脉高压大鼠右心室的表达。方法　SD大鼠 2 0只随机均分成正常对照组 (正常对照 )和低氧高二氧化碳 4周 (HH)组 ,分别测定平均肺动脉压力 (mPAP) ,右心室游离壁 (RV)和左心室加室间隔 (LV+S)的重量比 ;放免法测定大鼠血浆UⅡ含量 ;免疫组化方法检测心肌细胞、心肌小动脉UⅡ蛋白的表达 ;组织原位杂交方法检测心肌细胞、心肌小动脉UⅡmRNA和UⅡ受体 (UT)mRNA的表达。结果　 ( 1 )HH组mPAP和RV/LV +S比正常对照组分别高 5 2 .0 %与 2 5 .4% (P均 <0 .0 1 )。 ( 2 )HH组血浆UⅡ水平较正常对照组无明显增高 (P >0 .0 5 )。 ( 3)免疫组化显示HH组心肌细胞UⅡ蛋白表达阳性率和心肌小动脉UⅡ蛋白表达的平均吸光度值 ,均明显高于正常对照组 (P <0 .0 1 )。 ( 4 )组织原位杂交可见HH组的心肌细胞UⅡmRNA表达阳性率和心肌小动脉UⅡmRNA表达的平均吸光度值 ,均明显高于正常对照组 (P均 <0 .0 1 )。 ( 5 )组织原位杂交可见HH组的心肌细胞UTmRNA表达阳性率和心肌小动脉UTmRNA表达的平均吸光度值 ,均较正常对照组明显增高 (P均<0 .0 1 )。结论　慢性低氧高二氧化碳性大鼠肺动脉高压及右室肥大形成过程中 ,心肌小动脉和心肌细胞的UⅡ及其受体UT?更多还原

【Abstract】 Objective To observe the expression of Urotensin Ⅱ(UⅡ) and its receptor (UT) on right ventricle in rats with chronic pulmonary hypertension induced by hypoxia and hypercapnia. Methods Twenty male Sprague-Dawley rats were randomly divided into normal control group(NC) and hypoxia-hypercapnia 4-week group (HH). Mean pulmonary arterial pressure and the weight ratio of right ventricle (RV) to left ventricle plus septum (LV+S) were calculated separately. UⅡ in plasma was measured using radioimmunoassay. The expression of UⅡ was observed in right ventricle myocytes and right ventricle arteries by immunohistochemistry. The expression of UⅡ mRNA and UT mRNA were observed in right ventricle myocytes and right ventricle arteries by in situ hybridization. Results (1) The mPAP and RV/LV+S of HH group were higher respectively than those of NC group (P<0.01, respectively). (2) The plasma UⅡ content of HH group was not increased obviously than that of NC group (P>0.05). (3) The expression score of UⅡ,UⅡ mRNA, UT mRNA by right ventricle myocytes in HH group were higher significantly than those of NC group( P<0.01 respectively). (4)The average value of integral light density (LD) of UⅡ,UⅡ mRNA, UT mRNA by right cardial arteries in HH group were higher significantly than those of NC group ( P<0.01, respectively). Conclusion The expression of UⅡ in right ventricle arteries and right ventricle myocytes increase significantly during the formation of pulmonary hypertension and right ventricle hypertrophy in rats chronically exposed to hypoxia-hypercapnia. These changes indicate that UⅡ might be involved in right ventricle remodeling,which promotes proliferation of cardiac muscle cells.更多还原