【摘要】 目的　构建hIL15M -TNFαM融合毒素的表达质粒 ,为该融合毒素的功能研究及临床应用前景的分析评价奠定基础。方法　通过PCR删除hIL15 5′端前 8个氨基酸残基的编码序列获得人白介素 15突变体 (hIL15M ) ,将hIL15M基因片段与人工改造的TNFα基因按正确的阅读框架融合 ,定向克隆于表达载体pET16b。结果　经酶切分析 ,所构建的质粒均插入hIL15M -TNFαM融合基因。结论　利用 pET16b表达载体成功构建融合毒素hIL15M -TNFαM的表达质粒更多还原

【Abstract】 Objective To characterize the biological function and clinical perspective of hIL15M—TNFαM fusion toxin,a plasmid expressing this fusion molecule was constructed.Methods hIL15McDNA was obtained from plasmid pUC9NdeI-hIL15 by PCR.Artificially modified TNFαcDNA was fused precisly to the reading frame of hIL15Mand further cloned into the E.coli expression vector pET16b.Results The recombinant plasmid containing hIL15M—TNFαM fusion gene was obtained as demonstrated by restriction analysis. Conclusion We succeed in constructing the recombinant plasmid expressing hIL15M—TNFαM fusion toxin.更多还原