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RP-HPLC法测定不同方法加工天麻中天麻素含量

CONTENT DETERMINATION OF GASTRDIN IN DIFFERENTLY PROCESSED TIANMA BY RP-HPLC

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【作者】 袁晓袁萍尤敏斯缨王有为

【Author】 YUAN Xiao, YUAN Ping, YOU Min, SI Ying, WANG You-wei (Wuhan Institute of Botany, CAS,430074, Wuhan,Hubei,PRC)

【机构】 中国科学院武汉植物研究所中国科学院武汉植物研究所 430074湖北省武汉市430074湖北省武汉市

【摘要】 研究和评估了 3种加工新鲜天麻的方法 .方法 :新鲜天麻分别经水蒸汽法、1 2 5℃加热烘烤法和 6 0℃加热烘烤法后制成干天麻 ,用RP_HPLC法测定 .结论 :按 3种方法制备所得干天麻中的天麻素含量分别为0 .1 31 6 % ,0 .1 31 3%和 0 .0 2 70 % .实验结果分析表明 :水蒸汽法最适合中药材天麻的加工 ,有良好的外观 ;1 2 5℃加热烘烤法特别适合制药工业对天麻粉末的需要 ;6 0℃加热法是不可取的方法 .另外 ,及时加工是保持天麻素含量在天麻中稳定的一项关键措施

【Abstract】 Three processing methods of fresh Tian Ma (Rhizoma Gastrodiae) from traditional Chinese medicinal plant Gastrodia elata Blume were investigated and evaluated. Methods:To Mensurate and analyze the contents of gastrodin in Tian Ma samples processed by three methods of steaming on boiling water , roasting and heating at 125 ℃ and 60 ℃, RP-HPLC was used. Conclusion:The contents of gastrodin in processed Tian Ma were 0.1316%, 0.1313% and 0.0270%, determined by RP-HPLC, respectively. Experimental results: The processing method of steaming on boiling water was mostly suitable for merchandised Tian Ma, which owned a good appearance, roasting at 125 ℃ specially for a large scale of pharmaceutical usage of Tian Ma powder, the processing method of heating at 60 ℃ was not a good option for processing fresh Tian Ma. Additionally, instantly processing in any processing method was an important requirement of keeping the content of gastrodin in Tian Ma stabilizing.

【关键词】 天麻天麻素加工方法RP-HPLC法含量
【Key words】 Gastrodia elata blumeGastrodinprocessing methodRP-HPLCcontent
【基金】 中国科学院武汉植物园“知识创新工程”所长基金资助 ( 0 2 0 3 5 10 9)
  • 【文献出处】 曲阜师范大学学报(自然科学版) ,Journal of Qufu Normal University(Natural Science) , 编辑部邮箱 ,2004年03期
  • 【分类号】R284.2
  • 【被引频次】10
  • 【下载频次】300
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