【摘要】 以双功能褐藻胶裂解酶 (Pseudoalteromonas sp.strain No. 2 72 )分别降解均聚古罗糖醛酸(PG)和均聚甘露糖醛酸 (PM) ,经 Bio- Gel- P6和 POROS- HQ2 0分离得到 2种三糖纯品 ,经 ESI- MS,1 H- NMR,1 3 C- NMR,1 H- 1 H COSY,1 H- 1 3 C HMQC确定其结构为 Δ4,5-古罗糖醛酸 - α(1→ 4 ) - L-古罗糖醛酸 -α(1→ 4 ) - L -古罗糖醛酸 (简写为Δ GG)和Δ4,5-甘露糖醛酸 -β (1→ 4 ) - D-甘露糖醛酸 -β (1→ 4 ) - D-甘露糖醛酸 (ΔMM) ,为进一步从事构效关系研究提供结构信息。更多还原

【Abstract】 Two kinds of unsaturated trisaccharides were prepared by digesting polyguluronate (PG) and polymannuronate (PM) with bifunctional alginate lyase, which purified from Pseudoalteromonas sp. strain No. 272. The trisaccharides were purified on Bio-Gel-P6 and POROS-HQ20 column, respectively. Using IR, ESI/MS, 1H-NMR, 13C-NMR, 1H-1H COSY and 1H-13C HMQC techniques characterized the structure. The structure of unsaturated triguluronate is Δ4,5-guluronate-α -(1→4)-L- guluronate-α- (1→4)-L- guluronate (ΔGG) and unsaturated trimannuronate is Δ4,5-mannuronate-β-(1→4)-D- mannuronate -β -(1→4)-D- mannuronate (ΔMM). The structural character of these marine acidic oligosaccharides can further be used in structure-activity relationship studies.更多还原