【摘要】 目的 :探讨一氧化氮 (NO)和诱导性一氧化氮合酶 (iNOS)在实验性大鼠腹主动脉瘤形成中的作用和机制。方法 :建立大鼠腹主动脉瘤灌注模型 ,对照组予以生理盐水腹主动脉灌注 ,余下予以猪胰弹性蛋白酶灌注制作腹主动脉瘤模型 ,并分为实验组 (术后腹腔注射生理盐水 )、药物组 (术后腹腔注射氨基胍 ) ,观察各组大鼠血清NO、腹主动脉瘤壁组织学和基质金属蛋白酶 9(MMP 9)、iNOS的变化。结果 :生理盐水灌注组大鼠术前术后血清NO无明显变化 ,iNOS、MMP 9表达弱阳性或阴性 ,动脉壁炎性细胞浸润轻、弹性蛋白降解少 ;实验组血清NO显著升高 ,iNOS及MMP 9表达强阳性 ,炎性细胞浸润重、弹性蛋白几乎完全降解 ;应用氨基胍后iNOS变化不明显 ,而NO显著降低 ,MMP 9表达显著减弱 ,炎性细胞浸润和弹性蛋白降解明显减轻。结论 :iNOS、MMP 9的表达和血清NO水平在实验组均显著升高。iNOS及其合成的NO能促进腹主动脉壁炎性细胞的浸润、中膜基质的降解和MMPs的表达 ,从而导致了腹主动脉瘤的生成更多还原

【Abstract】 Objective:To investigate the effects of nitric oxide(NO) and inducible nitric oxide synthase (iNOS) in the experimental abdominal aortic aneurysm (AAA) rat model.Methods:An intra-aortic elastase infusion model was used.Control rats received intra-aortic saline infusion.In the remaining groups,intra-aortic elastase infusion was used to induce aneurysm formation.These rats were treated with intraperitoneal injections of saline postoperatively(experimental group),aminoguanidine postoperatively(medicine group).Serum NO and aortic diameter were measured,Changes of histology,iNOS and MMP-9 were observed in the aortic wall.Results:Experimental group produced AAAs with significant production of iNOS,MMPs and serum NO compared with controls.In medicine group reduced aneurysm size and displayed suppression of MMPs expression,inflammatory infiltrates and serum NO production were detected.Conclusion:Expression of iNOS and MMP-9 are induced and serum NO levels are increased in experimental AAA,iNOS and NO production by iNOS play an important role with detrimental effects during experimental aneurysm development.更多还原