【摘要】 采用薄层层析、紫外吸收光谱以及气谱-质谱联用的方法分析了苍白杆菌Ochrobacterumsp.B2降解甲基对硫磷的中间产物。结果表明,B2水解甲基对硫磷产生对硝基苯酚(PNP),PNP通过产生4-硝基邻苯二酚(4-nitrocatechol,4-NC)和1,2,4-苯三酚(1,2,4-benzenetriol)的途径进一步代谢。苍白杆菌B2可以以PNP和4-NC为碳源生长,在菌的初始OD600值为0.02时,B2可在48h内将50mg/L的PNP完全降解,在60h内,将30mg/L的4-NC完全降解。更多还原

【Abstract】 The parathion-methyl degradation by Ochrobacterum sp. B2 was analyzed by thin layer （chromatography,） UV spectroscopy and gas chromatography-mass spectrometry analysis. The （results） indicated that parathion-methyl was hydrolyzed to p-nitrophenol, and this metabolite was further （degraded） to 4-nitrocatechol and 1,2,4-benzenetriol. B2 could grow on a medium with p-（nitrophenol） or 4-nitrocatechol as a sole carbon source. 50 mg/L p-（nitrophenol） was degraded （completely） in 48 h and 30 mg/L 4-nitrocatechol disappeared almost in 60 h when the （initial） OD₆₀₀ of B2 cells was 0.02.更多还原