【摘要】 为开发防治番茄灰霉病Botrytiscinerea的生防细菌,进行了枯草芽孢杆菌BacillussubtilisBS-208和BS-209菌株对番茄灰霉病的温室和田间防治试验,并测定了两菌株对番茄灰霉病菌的抑制作用。结果表明:BS-208菌株分泌物对番茄灰霉病菌菌丝生长的抑制率为44%以上;BS-208和BS-209菌株的发酵液在经稀释10倍后,对番茄灰霉病菌分生孢子萌发的抑制率可达90%以上。经温室盆栽试验测定,以BS-208和BS-209菌株发酵液和菌体处理后24和48h接种病菌,防效均达75%以上,好于0h接种的防效,但分泌物滤液防治效果较差。两年的田间试验结果表明,BS-208和BS-209制剂对番茄灰霉病均具有良好的防治效果,其防效随浓度加大而提高,以800倍液的防效最好,两菌株制剂800倍液连续3次施药后防效均达到74%以上,并且BS-208菌株的防效略高于BS-209菌株。更多还原

【Abstract】 To exploit the antagonistic bacteria against Botrytis cinerea, experiment was conducted for controlling tomato gray mold by Bacillus subtilis BS-208 and BS-209 in the greenhouse and field. Inhibition effect to conidia germination and mycelium growth were also assayed. The (secretion) of B.subtilis BS-208 inhibited over 44% the mycelium growth of B.cinerea. (Fermentation) liquor of strains BS-208 and BS-209 inhibited above 90% conidia germination after diluting (10 fold.) The greenhouse experiment showed that the fermentation liquor and spore of BS-208 and BS-209 had above 75% control efficacy with inoculating after 24 and 48 h, but the (secretion) (exhibited) bad control efficacy. Two years experiment in the field indicated that the (formulation) of B. subtilis BS-208 and BS-209 could effectively control tomato gray mould. With the increasing of concentration its control efficacy became better and 800-fold treatment had the best control (efficacy.) After three times treatment with the 800-fold formulation continuously, the control (efficacy) reached above 74% and the effect of BS-208 was better than that of BS-209’s.更多还原