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几种苯甲酸类化合物对甜菜夜蛾多酚氧化酶活性的影响

The Effects of Benzoic Acid Compound Series on PPO of Spodoptera exigua Hübner

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【作者】 高兴祥罗万春于天丛王树栋丁琦赵淑英

【Author】 GAO Xing-xiang~1,LUO Wan-chun~(1*),YU Tian-cong~1, WANG Shu-dong~1,DING Qi~1,ZHAO Shu-ying~2Technique, Tai’an 271018,China; 2. Chemical Academy, Jinan University, Jinan 250022,China)

【机构】 山东农业大学农药毒理与应用技术省级重点实验室济南大学化学化工学院 山东泰安271018山东泰安271018山东济南250022

【摘要】 为了探讨环境友好杀虫剂的开发途径,以对羟基苯甲酸等5种苯甲酸类化合物为效应物,研究了该类化合物对甜菜夜蛾多酚氧化酶(PPO)活性的影响。结果表明:5种化合物均对该酶表现出一定的抑制作用,其中以对羟基苯甲酸的抑制作用最为显著,I50为0.23mg/mL,其次为5-甲氧基水杨酸。进一步研究这两种化合物对甜菜夜蛾多酚氧化酶的抑制机理,发现对羟基苯甲酸属于可逆反应的混合型抑制剂,其Ki与Kis分别为0.46mg/mL和0.02mg/mL;5-甲氧基水杨酸属于竞争性抑制剂,Ki为0.87mg/mL。

【Abstract】 The effects of 5 benzoic acid compounds on polyphenol oxidase(PPO) of Spodoptera (exigua) were studied, when they were partially purified by 40% saturated (NH42SO4 and Sephadex G-100 gel filtration. The PPO is a key enzyme which takes part in melanin formation, sclerotization, recognition system in the development of insect, The results indicated that (p-hydrobenzoic) acid was the strongest inhibitor with I50 0.23 mg/mL, and the second compound was 5-methoxysalicylic. In addition, the results also showed that the PPO activity was inhibited by p-hydrobenzoic acid through mixed-competition with Ki=0.46 mg/mL,Kis=0.02 mg/mL,and 5-methoxysalicylic was competitive inhibitor with Ki=0.87 mg/mL.

【基金】 国家自然科学基金资助项目(30270887).
  • 【文献出处】 农药学学报 ,Chinese Journal of Pesticide Science , 编辑部邮箱 ,2004年01期
  • 【分类号】TQ450
  • 【被引频次】25
  • 【下载频次】246
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