【摘要】 苹果酸脱氢酶普遍存在于各种生物中,它负责催化草酰乙酸和苹果酸之间的相互转换。根据其辅酶的特异性和在细胞内的分布和生理功能的不同,苹果酸脱氢酶在高等植物中可以区分出不同的类型。依赖于NAD的细胞质型苹果酸脱氢酶(cyMDH)是其中研究较少的一类。报道了从小麦(TritiumaestivumL.)中克隆的一种cyMDH的部分cDNA序列,并命名为W-MDH1。进化树分析表明来自不同生物的MDH可能是通过基因复制形成的。W-MDH1在小麦的根、茎、叶中呈组成型表达。更多还原

【Abstract】 Malate dehydrogenase (MDH) is ubiquitous in nature, which catalyzes t he interconversion of oxaloacetate and malate. Higher plants contain multiple fo rms of MDHs that differ in co-enzyme specificity, subcellular localization and p hysiological functions. Cytosolic NAD-dependent MDH (cyMDH) is one class of MDH that is still less investigated. Here we report the isolation and characterizati on of a partial cDNA clone encoding cyMDH from wheat, namely W-MDH1. The phyloge netic relationships among MDHs from different organisms showed that multiple for ms of MDHs might be evolved through gene duplication of the pre-existing nuclear gene. Expression studies demonstrated that W-MDH1 gene was constitutively trans cribed in the leaf, stem and root tissues.更多还原