【摘要】 本研究旨在提取黑龙江产高粱外种皮中的原花青素并鉴定其组成。通过正交试验对提取工艺进行优化,采用柱层析、葡聚糖(SephadexLH-20)凝胶色谱进行纯化和分离,纯化和分离的原花青素产物用ESI-MS鉴定其组成。在影响提取率的4个因素温度、料液比、时间和pH值中,温度对提取率的影响最为显著,料液比也有一定影响。研究得到最佳提取工艺为:温度80℃,时间1.5h,pH值为4,料液比为1∶20;通过ESI-MS鉴定出了原花青素的组成成分的分子量,黑龙江产高粱外种皮中的原花青素以聚合度小于5的低聚体为主。更多还原

【Abstract】 In this study, procyanidins were extracted and determined from the episperm of Sorghumseed harvested in Heilongjiang Province. Extraction conditions were optimized by orthorhombic analysis. Column chromatography and gel (Sephadex LH-20) chromatography were conducted for further separation and purification. The resulting purified and separated extracts were identified by using ESI-MS. Among the four extraction factors concerned, including temperature, ratio of material/solution, extraction time and pH value, temperature was the most significant that affected the extraction rate, followed by the ratio of material/solution. Molecular weights of various procyanidins extracted were measured by ESI-MS. The optimized extraction condition was to extract at temperature 80℃ for 1.5 h at pH 4, and with a 1∶20 material/solution ratio. In the material studied, oligomers, no larger than pentamers, were the major procyanidins presented in the Episperm of Sorghumseed.更多还原