【摘要】 研究了菌体生长、产物合成及底物消耗的最佳关系,通过5L实验罐的正交试验,确定了以兽疫链球菌NUF—036分批发酵生产透明质酸的工艺条件:通风量2.0L/min,搅拌转速600r/min,pH=7.5,温度35℃.在此条件下,透明质酸的产量为3.7g/L,分子质量为3.8×106Da.建立了兽疫链球菌NUF—036分批发酵生产透明质酸的动力学模型.更多还原

【Abstract】 Optimum relationship of substance consumption, biomass growth and products synthesizes was studied in this paper. The optimum batch fermentation condition in (5 L) fermentor was that the aerobic rate, 2.0 L/min, the agitation rate, 600 r/min, pH =7.5, temperature, 35 ℃ through orthogonal design, in which the molecular weight of hyaluronic acid (HA) was 3.8×10~6Da,and HA concentration increased to 3.7 g/L. The model of Streptococcus zooepidemicus NUF—036 in batch fermentation was established in this paper.更多还原