节点文献
黄单胞菌α-淀粉酶基因的克隆、测序及表达探讨
Cloning,sequencing and expression of α-amylase gene from Xanthomonas campestris pv.cmpestris 8004
【摘要】 用鸟枪法构建了野油菜黄单胞菌(Xanthomonascampestrispv.campestris)8004的基因组文库,经LBSP法筛选,得到了含α 淀粉酶基因的基因片段,SDS PAGE凝胶电泳确定其蛋白质分子量约为45kd;经测序后,分析其序列结构并翻译成氨基酸序列.
【Abstract】 The genomic library of Xanthomonas campestris pv.campestris 8004 was constructed by shot gun,and positive cloning of α-amylase was screened with LBSP method.The weight of this protein was determined to be about 45 kd by SDS-PAGE.α-amylase gene was sequenced and translated into amino acid after sequence structure analysis.
【关键词】 黄单胞菌;
α淀粉酶基因;
克隆;
测序;
表达;
【Key words】 Xanthomonas campestris; α-amylase gene; clone; sequence; expression;
【Key words】 Xanthomonas campestris; α-amylase gene; clone; sequence; expression;
【基金】 河南省重点科技攻关项目(0123011700)
- 【文献出处】 河南农业大学学报 ,Journal of Henan Agricultural University , 编辑部邮箱 ,2004年04期
- 【分类号】Q785
- 【被引频次】6
- 【下载频次】131