【摘要】 研究了反义Trx＿S基因对小麦Trx＿h基因表达抑制情况,对两个转反义Trx＿S基因品系(00TY5和00T89)的T3代进行了PCR检测,证明反义Trx＿S基因已经遗传到转基因品系T3代植株当中.对不同成熟时期的转基因品系种子的α＿淀粉酶和β＿淀粉酶活性测定表明,转基因种子在不同成熟时期的α＿淀粉酶和β＿淀粉酶活性有较大的变化,但与对照相比均有不同程度的降低,其中α＿淀粉酶活性最低值出现在花后33～36d,平均降低幅度达到61%;而β＿淀粉酶活性最低值出现在花后22d.方差分析表明不同成熟时期的差异均达到显著或极显著水平,表明反义Trx＿S基因对小麦Trx＿h基因的表达具有明显抑制作用.更多还原

【Abstract】 In order to study the expression inhibition of thioredoxin h （Trx_h） gene in transgenic wheat seeds,two transgenic lines（00TY 5 and 00T 89） were analyzed by PCR.The result showed that anti_trxs gene had been inherited to T₃ generation.The results of amylase activity analysis of transgenic seeds and non_transgenic ones in different seed maturities indicated that α_amylase and β_amylase were lowered distinctly in transgenic seeds,although the activity varied greatly in different maturities.The lowest α_amylase activity was 33～36 d after florescence,while the lowest β_amylase activity was 22 d after florescence.Variance analysis indicated that the variances in different maturities were all significant or remarkably significant at 0.01 level.The results showed that anti_trxs gene inhibited the expression of Trx_h gene in transgenic wheat seeds.更多还原