【摘要】 以悬铃木组织培养苗叶片为材料,比较了提取叶片DNA的4种方法:SDS-Ⅰ,SDS-Ⅱ,CTAB-Ⅰ,CTAB-Ⅱ,并用紫外光谱吸收、凝胶电泳、限制性内切酶酶切、RAPD等方法进行鉴定.研究结果表明,4种方法提取的DNA的得率约为0 347～0 518μg·mg-1,分子量约为23kb,均可被酶切.此外,CTAB-Ⅰ和CTAB-Ⅱ提取的DNA可用于RAPD分析.综合考虑得率、纯度等因素,作者认为CTAB-Ⅱ法可作为悬铃木叶片DNA提取的最佳方法.更多还原

【Abstract】 This paper dealt with extraction of DNA from Platanus orientalis leaves with 4 methods:SDS-Ⅰ, SDS-Ⅱ, CTAB-Ⅰand CTAB-Ⅱ,and they were tested with ultraviolet spectrophotometer anaylis, agarose gel electrophoresis, restriction enzyme digestion and RAPD reaction respectively. The results indicated that 3.47～5.18(μg·mg^-1) DNA was obtained from the fresh leaves,their molecular weights were about 23 kb,and they were all suitable for restriction enzyme digestion. Moreover, DNA ebtained with CTAB methods might be used for RAPD analysis. All fectors considered,CTAB-Ⅱ was considered as one of the best methods for extraction of DNA from Platanus orientalis leaves.更多还原