【摘要】 目的 :在基因表达水平上研究黏附分子 ( E-cadherin,CD44v6)与卵巢癌转移的关系。方法 :用免疫组化 S-P法 ,选取 1 997～ 2 0 0 2年间石蜡包埋的卵巢上皮性肿瘤组织块 1 0 7块 ,其中良性上皮性肿瘤 3 0例 ,交界瘤 1 3例 ,恶性 3 4例 ( 、 期 4例 , 、 期 3 0例 ) ,对 3 0例恶性晚期 ( 、 期 )病例选取对应的大网膜转移组织。结果 :3 0例良性上皮性肿瘤组织中 ,E-钙黏附蛋白 1 0 0 %强阳性表达 ,CD44v61 0 0 %阴性。 1 3例交界瘤中 ,E-钙黏附蛋白有 1 1例 ( 84.6% )强阳性 ,CD44v6有 1例弱阳性表达。 3 4例恶性上皮性肿瘤中 ,E-钙黏附素有2 4例 ( 70 .6% )阳性表达 ,CD44v6有 1 1例 ( 3 2 .4% )阳性表达。二指标在良性上皮性肿瘤与卵巢癌之间的阳性表达有显著性差异 ( P =0 .0 0 1 ,P =0 .0 0 0 )。二指标在原发灶和大网膜转移灶之间表达无显著差别 ( P =1 .0 0 0 ,P =1 .0 0 0 )。结论 :E-cadherin及 CD44v6在上皮性卵巢肿瘤中的表达呈相反趋势 ,E-cadherin及 CD44v6与卵巢癌组织学分级及预后无明显相关性更多还原

【Abstract】 Objective: To study the relationship of twe adhesive molecules (E-cadherin and CD44v6) with human ovarian carcinoma and the relation between the two markers. Methods: Immunohistochemically examined expression of E-cadherin and CD44v6 in formalin-fixed paraffin-embedded archival specimens of primary human ovarian carcinoma from patients undergoing curative surgery. 107 samples of epithelial ovarian neoplasms were included in this study (30 benign epithelial tumors, 13 borderline epithelial tumors, and 30 primary carcinomas and matched metastatic sites, 4 early stage carcinomas). Results: In 30 benign cases, E-cadherin is 100% positive, CD44v6 is 100% negative. In 13 borderline cases, E-cadherin is 84.6% positive, CD44v6 is 1/13(7.7%) weakly stained. In 34 malignant cases, E-cadherin positive expression is 24/34(70.6%), CD44v6 positive expression is 11/34(32.4%).There is no significant change about the expressions of E-cadherin and CD44v6 in primary and matched metastatic sites. Furthermore, the expression of E-cadherion and CD44v6 have no relationship with tumor differentiation and survival. Conclusion: E-cadherin and CD44v6 are involved in the tumorigenesis and progression of epithelial ovarian cancer, they play an important role in metastasis of ovarian cancer. E-cadherin and CD44v6 have opposite trend in the expression of epithelial ovarian neoplasms. Their expressions have no correlaton wih histological grade and survival.;更多还原