【摘要】 以鄂尔多斯高原碱湖的钝顶螺旋藻S1、鄂尔多斯螺旋藻S2、引进的钝顶螺旋藻S3和极大螺旋藻S4为材料,采用两种方法分别测定了其DNA和RNA的含量。结果如下:改良二苯胺法测定的DNA含量为1.03μg/mgDW～1.68μg/mgDW;紫外分光光度法测定的为5.86μg/mgDW～10.03μg/mgDW。两种方法测定的DNA含量排列均为S2>S4>S1>S3。苔黑酚法测定的RNA含量为14.36μg/mgDW～22.56μg/mgDW;紫外分光光度计法测定的为17.67μg/mgDW～39.53μg/mgDW。两种方法测定的RNA含量排列均为S2>S1>S3>S4。无论DNA还是RNA,紫外分光光度法测定的值高。更多还原

【Abstract】 The content of DNA and RNA in Spirulina（Arthrospira）platensis（S₁）,S.（A.）erdosensis（S₂）from alkaline lake in Erdos Plateau and the introduced S.（A.）platensis（S₃）and S.（A.）maxima（S₄）were determined with two methods respectively. The results showed that the content of DNA in Spirulina determined by means of ameliorative diphenylamine was 1.03～1.68μg/mgDW;That determined by ultraviolet absorption was 5.86～10.03μg/mgDW. Both methods showed following tendency:S₂>S₄>S₁>S₃.The content of RNA in Spirulina determined by means of 3,5-Dihydroxytoluene was 14.36～22.56μg/mgDW.That determined by ultraviolet absorption was 17.67～39.53μg/mgDW. Both methods showed following tendency:S₂>S₁>S₃>S₄.The content whether DNA or RNA determined by means of ultraviolet absorption is higher.更多还原