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苜蓿花叶病毒复制酶(P2亚基)基因3’端cDNA植物表达载体的构建
CONSTRUCTION OF PLANT EXPRESSION VECTOR FOR THE 3’ TERMINAL cDNA OF ALMV REPLICASE (SUBUNIT P2) GENE
【摘要】 将克隆的苜蓿花叶病毒中国分离株(AlMV-Ch)复制酶(P2亚基)基因3’端约1/2序列及其3’端非编码区(1234bp)cDNA重组于植物表达载体pROKⅡ中,构建了其正链RNA的表达载体pAMR3T,为进一步开展转基因研究创造条件。
【Abstract】 The cloned 3’terminal cDNA fragment of Alfalfa Mosaic Virus Chinese isolate(AlMV-Ch ) was constructed into plant transformation binary vector pROKII, the results of restriction enzyme analysis and PCR amplification showed that plant expression vector pAMR3T was made.
【关键词】 苜蓿花叶病毒;
复制酶(P2亚基)基因;
植物表达载体;
【Key words】 Alfalfa mosaic mirus (AlMV); Replicase (subunit P2) gene; Plant expression vector;
【Key words】 Alfalfa mosaic mirus (AlMV); Replicase (subunit P2) gene; Plant expression vector;
【基金】 国家自然科学基金资助项目(39860042)
- 【文献出处】 内蒙古农业大学学报(自然科学版) ,Journal of Inner Mongolia Institute of Agriculture and Animal Husbandry , 编辑部邮箱 ,2004年01期
- 【分类号】S432
- 【被引频次】1
- 【下载频次】59