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一种新的测定生物体系中过氧化脂质(LPO)的改进碘量法的建立
The modified iodometric method for lipid peroxide(LPO)assay in biological samples
【摘要】 目的 :为方便而准确测定生物体系中的过氧化程度。方法 :用改进碘量法对LDL、培养细胞的上清液、人血浆的脂质氧化水平测定 ,并经四种质量控制指标测定。结果 :改进碘量法具有快速、简便、灵敏、准确等特点 ,易在一般实验室推广。结论 :改进碘量法是对生物体系中脂质氧化水平测定在方法学上的一项重要补充
【Abstract】 Objective:In order to measurement Lipid peroxide(LPO)easily and exactly in normal laboratories.Methods:we adopt the modified iodometric method to assayment in LPO.we have measurement LPO of LDL,endothlium-condition medium and human plasma,and four quality control indexes,including standard curve,accuracy,precision and stability were investigated. Results:The results indicated that this method has good accuracy,sensitivity,precision,stability,and be easily practice by normal laboratories.Conclusion:It should be an important supplement to the methodology of LPO measurement.
【Key words】 Lipid peroxide(LPO); Modified iodometric assay; Biological sample;
- 【文献出处】 农垦医学 ,Agricultural Reclamation Medicine , 编辑部邮箱 ,2004年02期
- 【分类号】R446.1
- 【下载频次】110