【摘要】 从长期受苯胺污染环境中筛选到一株细菌NKS,能以苯胺为唯一的碳源、氮源生长.NKS降解苯胺的最适温度和pH分别为30℃和7.2,最适苯胺浓度为3000mg／L.NKS还可利用邻苯二酚和邻甲苯胺.提高接种浓度对NKS的降解效果有显著影响,重金属离子对NKS的生长和苯胺降解均有不同程度的抑制作用,以Ag+,Hg+最明显.对NKS与苯胺降解代谢有关酶类的测定结果表明,NKS中催化邻苯二酚开环的酶主要为邻苯二酚-2,3-双加氧酶(CD230),且该酶为诱导酶.PCR结果表明NKS中与苯胺酶降解有关酶的基因位于细菌的染色体上.更多还原

【Abstract】 An smiline-degrading bacteria NKS, which was able to grow on aniline as sole carbon, nitrogen and energy sources , was isolated from the nature. NKS was capable of growing on aniline medium higher than 5000mg/L. The optimun temperature and pH value for growth and aniline degradatopm were 30℃ and 7. 2, respectively. The best concentration of aniline was 3000ng/L. NKS could also use catechol and o-Toluidine as carbon, nitrogen and energy sources. but could not use Other substituted aniline compounds. A higher inoculation of bacterium could result a better degradation effect for NKS. Heavy metal ions could inhibit the growth of NKS and the degradation of aniline, especially Ag+ and Hg+. NKS degraded aniline via a meta-cleavage pathway. Only the cells growing on aniline contained catechol-2, 3-dioxygenase, which showed that catechol-2,3-dioxygenase was an inducible enzyme for NKS. PCR results indicated that genes responsible for the degradation are located on bacterial chromoaome.更多还原