【摘要】 目的 :研究穿心莲内酯对免疫功能的影响 ,探索其发挥作用的免疫机制。方法 :采用生物活性法和ELISA法检测用有效成分为穿心莲内酯的莲必治注射液处理人外周血单个核细胞上清中的IFN α、IFN γ、TNF α、IL 8含量 ;用单核巨噬细胞巨噬鸡红细胞来研究其促吞噬功能以及用LDH释放法检测其对NK细胞杀伤活性的影响。结果 :和空白对照组比 ,莲必治注射液作用外周血单个核细胞能显著诱导IFN α、IFN γ、TNF α产生 (P <0 .0 5 ) ,但对IL 8诱生无明显影响 ,甚至轻度抑制。莲必治注射液能提高豚鼠腹腔单核巨噬细胞对鸡红细胞的吞噬百分率 (P <0 .0 5 )及提高人外周血中NK细胞杀伤K5 6 2细胞的活性 (P <0 .0 5 )。结论 :穿心莲内酯是一种具有调节机体非特异免疫功能的免疫刺激剂 ,通过对NK、MΦ及细胞因子分泌的影响而发挥免疫调节作用更多还原

【Abstract】 Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) culture supernatants dealt with by different concentrations of LianBiZhi (LBZ) injection, the effective component of which is andrographolide, were detected by biological activity test or ELISA in vitro. The effects of LBZ injection on macrophage phagocytotic function and natural killer cells cytotoxicity were examined by means of macrophage to phagocytize cock erythrocyte and measurement of lactate dehydrogenase (LDH) activity released from the damaged cells, respectively.Results: The LBZ injection could not only enhance the phagocytosis activity of peritoneal macrophage from guinea pig to phagocytosis cock erythrocytes, but also augment the cytotoxicity mediated by natural killer cells from PBMCs.Conclusion: Andrographolide is an immunostimulant agent which can modulate both antigen specific and nonspecific immune function by means of its natural killer cells, macrophage and cytokines.更多还原