【摘要】 首次报道了吸附在活性炭表面的酪氨酸酶 (Tyr)和漆酶 (Lac)能进行准可逆的直接电化学反应 ,并保持对氧还原的电催化作用 .本文所用的固定酶的方法具有简单、易于操作和酶活性保持良好等优点 ,可用于研究氧化还原蛋白质和酶的直接电化学和用于制备生物燃料电池的催化剂更多还原

【Abstract】 It is reported for the first time that tyrosinase or laccase, adsorbed on the surface of active carbon powder can undergo the quasi-reversible direct electrochemical reaction and have the electrocatalytic activity for the O₂ reduction. The formal redox potentials of tyrosinase and laccase are -0.138 and -0.161?V, respectively. The direct electrochemical reactions of tyrosinase and laccase can not occur at the glassy carbon electrode. These results indicated that the microenvironment constructed with active carbon and Nafion membrane is favorable for the direct electrochemical reactions of biomacromolecules. The immobilization method has several advantages, such as simplicity, easy to operation and keeping good activity of enzyme etc. Therefore, this method can be used for investigating the direct electrochemistry of redox proteins and enzymes. It may be also used to prepare the catalysts for biofuel cells.更多还原