【摘要】 根据大豆疫霉及其近缘卵菌ITS序列差异设计了一对寡聚核苷酸引物 ,用于对包括大豆疫霉在内的 2 0种真菌的PCR检测。结果表明 :在优化的反应体系及扩增条件下 ,该对引物只在大豆疫霉为模板的扩增体系中具有一 330bp的扩增产物 ,表现出强特异性。利用该特异引物可稳定地从含有大豆疫霉游动孢子或卵孢子的土壤及其发病组织中检测出病原菌。该检测方法对大豆疫霉游动孢子和卵孢子的检测理论精度可达 0 5个孢子更多还原

【Abstract】 Based on the sequence of internal transcribed spacer regions(ITS)of the ribosomal gene,a couple of specific primers for P sojae was synthesized,and a single band of PCR product about 330 bp was amplified from the crashed sample of zoospore or oospores carried in the soil or the diseased soybean tissues.The protocol was determined to efficiently detect pathogen at a theoretical rate of half spore.更多还原