【摘要】 应用基因克隆技术将黑曲霉产糖化酶基因cDNA转入经优化的受体菌(酿酒酵母京龙JL108号),获得多株具有产糖化酶能力的酿酒酵母,再经反复筛分、驯化获得JL1(YIp128D.17N),采用变性PVA固定化增殖活细胞包埋技术,将JL1制成具有糖化酒化“双功能”的固定化酵母,大幅度提高细胞数,增强了大生产基因工程菌的糖化、酒化能力,载体产酶能力在10u/g·h以上,酒精发酵醪液中酶活达20u/ml以上,在年产5000t的富川县酒精厂进行工业性生产应用试验,技术指标完全达到并超过传统工艺的生产水平,淀粉出酒率达55%以上。更多还原

【Abstract】 The gene cDNA of aspergillus niger(wh ic h produced saccharifying enzyme)was transferred into the optimized acceptor yea st(liquor-making yeast Jinglong JL108)by genic clone techniques.As a resu lt,multiple strains of liquor-making yeast in posses-sion of saccharifying en zyme-producing capability were obtained.After repetitive selection and domesti cation,the strain of JL1(YIp128D.17N)was obtained.Then JL1was made i nto immobilized yeast in possession of"double functions"including saccharifyin g capability and alcoholizing capability by the technique of denaturing PVA immo bilized proliferation living cell embedment.The tech-nique could greatly impro ve cell amount and strengthen saccharifying capability and alcoholizing capabili ty of genetic engineering yeast in mass production.The enzyme-producing capabi lity of the carrier was above10u/g·h and the enzyme survivability in ferment ed liq-uid was above20u/ml.The trial production in Fuchuan Grain Distillery with annual alcohol yield as5,000tons proved that the tech-nical indexes had completely achieved or exceeded the corresponding indexes before.And the yi eld rate of amylum was above55%.(Tran.by YUE Yang)更多还原