【摘要】 用CMC糖化力法和纤维素减重法探讨了对一组高效稳定的纤维素分解细菌复合系MC1的产酶条件。结果表明,在6种纤维素材料中,可直接利用的天然纤维素含量高的C源(如滤纸、棉花)存在下表现出高活性;利用蛋白胨和酵母粉作N源时的纤维素酶活性远高于硝酸铵、尿素等无机N源;以滤纸和酵母粉作为惟一C、N源时,最适发酵浓度分别为0.5%和0.125%,其产酶最高峰均出现在发酵第5d。MC1最适纤维素分解温度为55℃,但60℃以上的高温抑制MC1纤维素分解酶;MC1的最适氧气浓度为0.01～0.04mg·L-1,过高过低的氧气浓度均抑制纤维素分解。MC1的微好氧特点对堆肥工程降低生产成本具有重要意义。更多还原

【Abstract】 The conditions of cellulase production of microbial consortia MC1 capable of degrading cellulose with high efficiency were investigated with CMC saccharogenic powder and gravimetry. The microbial consortia MC1 could directly degrade carbon source with high native cellulose content (such as filter paper and cotton). It has been found that cellulase activity using peptone and yeast powder as nitrogen source was higher than that using inorganic nitrogen source, such as urea and ammonium nitrate. The optimum fermentation concentrations using filter paper and yeast powder as sole carbon and nitrogen source were found to be 0.5% and 0.125% , respectively, and the highest level of cellulase was discovered at the fifth day after inoculation. The optimum temperature for degrading cellulose was 55 ℃ , but the cellulase activity was inhibited when the temperature was over 60 ℃ .The optimum oxygen concentration of MC1 was 0.01～ 0.04 mg· L- 1 and either higher or lower oxygen concentration was not available to degradation of cellulose. It may be promising and important for the characteristic of microaerobe of MC1 in decreasing the cost of composting engineering.更多还原