【摘要】 采用斑马鱼和鲫鱼作为生物材料,分别研究了兽药添加剂阿散酸和土霉素对斑马鱼的急性毒性及对鲫鱼肾细胞DNA损伤。急性毒性试验表明,阿散酸和土霉素的斑马鱼96hLC50分别为520.747和1341.764mg·L-1。彗星试验结果表明,阿散酸引起的DNA损伤较小,与阴性对照相比无显著性差异(P>0.05);而土霉素能引起较大的DNA损伤,与阴性对照相比均有极显著性差异(P<0.01)更多还原

【Abstract】 Veterinary drug additives are widely used in the stock raising. To this respect they represent a potential risk for the animals of many species, including human. In this paper, the toxicology of arsanilic acid and oxytetracycline were studied by using fish acute toxicity test and single cell gel electrophoresis test (comet assay). The results of acute toxicity test to zebra fish (Brachydanio rerio) showed the 96h- LC50 of arsanilic acid and oxytetracycline were 520.7 and 1 341.7 mg· L- 1 respectively. The DNA breakage capacity of two test substances was also assessed with SCGE in crucian (Carassius auratus) kidney cells in vivo. The data indicated that no statistically significant was found between the groups exposed to arsanilic and the control groups. However, oxytetracycline could induce extremely significantly different (P<0.01) DNA damage at all the doses tested, compared with the controls.更多还原