【摘要】 目的　采用激光扫描共聚焦技术 ,检测大鼠海马干细胞分化的神经元内钙离子浓度 ,以期为神经干细胞进一步应用于细胞替代治疗提供理论依据。方法　分离培养新生鼠的海马干细胞 ,采用激光扫描共聚焦技术测定其分化的神经元内钙离子浓度 ,并与原代培养的海马神经元内的钙离子浓度进行比较。结果　大鼠海马干细胞分化的神经元平均荧光强度为 94 3.6 5± 89.33,相同条件下原代培养的大鼠海马神经元平均荧光强度为 713.94± 5 5 .5 9(t=1.5 5 ,P >0 .10 )。结论　大鼠海马干细胞分化的神经元已具有神经元的一些特征 ,其细胞内钙离子的分布与原代培养的海马神经元相似。更多还原

【Abstract】 Objective To measure the concentration of intracellular free Ca 2+( i) of neurons differentiated from rat hippocampal stem cells by confocal laser-scanning microscopy and provide some theoretical bases for the substituted therapy with neural stem cell transplantation.Methods Neonatal rat hippocampal stem cells were isolated and cultured in the culture medium without serum and the differentiation was initiated.Then i of the differentiated neurons was determined and compared with the primary cultured hippocampal neurons.Results The averaged fluorescence intensity of the neurons differentiated from neural stem cells was 943.65±89.33,while the averaged fluorescence intensity of the primary cultured hippocampal neurons was 713.94±55.59(t= 1.55,P> 0.10).Conclusion Under the present culture conditions,the neurons differentiated from rat hippocampal stem cells have possessed certain neuronal properties.更多还原