【摘要】 目的　SARS冠状病毒的核衣壳 (Nucleocapsid)蛋白 (N蛋白 )是病毒的主要结构抗原 ,重组N蛋白可用作抗原检测患者血清中相应抗体。方法　以纯化的目的蛋白N 1,N 2分别包被 96孔板 ,检测正常人群及患者血清中抗SARS病毒抗体。结果　检测SARS感染患者血清 ,N 1阳性检出率为 5 5 .6 8% ,N 2阳性检出率为 5 6 .82 % ,与华大基因试剂盒相比符合率分别为 90 .12 %和 87.6 5 %。结论　重组核衣壳蛋白可做为检测SARS抗体的抗原蛋白。更多还原

【Abstract】 Objective To evaluate the specific antibody in the serum of SARS infected patients using recombinant nucleocapsid protein. Methods The 96-well plates were coated with recombinant N-1 and N-2 proteins, respectively. The specific serum antibodies in normal controls and SARS infected patients were assayed by ELISA, respectively. Results The positive rates of N-1 protein and N-2 protein in SARS infected patients were 55.68% and 56.82%, respectively. The concordance rates of N-1 and N-2 with the Huada kits were 90.12% and 87.65%, respectively. Conclusion The recombinant nucleocapsid protein could be used as an antigen for SARS diagnosis.更多还原