【摘要】 目的:探讨顺铂诱导瘢痕细胞凋亡过程中细胞内游离Ca2+浓度和三磷酸肌醇(IP3)浓度的变化特点和意义。方法:选择顺铂诱导瘢痕细胞凋亡,采用光镜、电镜、琼脂糖凝胶电泳和流式细胞术检测凋亡。Fura-2荧光负荷技术测(Ca2+)i,竞争性蛋白结合法测定IP3浓度。结果:顺铂浓度为2μg/mL作用于瘢痕成纤维细胞24小时出现典型的凋亡改变。瘢痕细胞凋亡过程中,细胞内(Ca2+)i明显升高,以凋亡早期更为明显。瘢痕细胞凋亡早期,IP3浓度明显升高,晚期IP3浓度明显下降。结论:在瘢痕细胞凋亡过程中,(Ca2+)i表现为上调趋势。IP3浓度在凋亡早期,表现为上调趋势,在稍晚阶段,表现为下调趋势,这为治疗瘢痕提供了新靶点。更多还原

【Abstract】 Objective To study the change and significance of intracellular-free calcium levels and inositol 1,4,5-trisphosphate levels in the process of scar cell apoptosis induced by cisplatin. Methods Apoptosis induced by cisplatin in scar cell was investigated by light and electronmicroscopy, agarose gel electrophoresis and flow cytometry. (Ca2+)i was determined by Fura-2 fluorescin load technic, IP3 was determined by competitive protein binding method. Results When scar cell were treated with cisplatin(2μg/ml)for 24 hours changes appeared typical of apoptosis. (Ca2+)i and IP3 were significantly increased, especially at the initial stage of apoptosis. However, from 2 to 24 hours after cisplatin treatment,IP3 levels progressively decreased, being significantly lower than those of the untreated controls. Conclusion In the process of cisplatin induced apoptosis of scar cell, (Ca2+)i and IP3 levels are up-regulated at the very initial stage but down-regulated from 2 hours afterwards.更多还原