【摘要】 为了培育抗穗发芽的种质资源,以13个小麦品种(品系)的植株体为受体材料,用花粉管通道及子房注射法进行了硫氧还蛋白反义基因(Anti-TrxS,与穗发芽抗性有直接关系)的遗传转化。花粉管通道法共处理小花2036朵,收获T0代种子1616粒,结实率为79.4%,将T0代种子大田点播成株行,T1代共出苗1424棵,出苗率为88.1%。对T1代苗按品种和处理组合的方式进行抽样检测,抽样株系取10个单株分别提取叶片基因组DNA,然后将单株叶片DNA等量混合,进行株系基因组DNA的PCR检测。检测结果为31个株系中有16个株系呈阳性反应。子房注射法共转化豫麦49、豫麦70、豫麦18、郑新991和95519等5个小麦品种(品系)的小花1206朵,收获种子89粒,结实率为7.4%,T1代共出苗41棵,出苗率为46.1%。对郑新991T1代株系(12棵幼苗的叶片基因组DNA混合样)进行PCR检测,电泳结果呈阳性反应。更多还原

【Abstract】 Plants of 13 wheat cultivars were used as receptors. Anti-TrxS gene was transferred into 2036 (florets) of receptors through pollen tube pathway. 1 616 seeds were harvested from transformed receptors with a seedsect ratio of (79.4%) and 1 424 plantlets were (obtained) from 1 616 seeds with a seedling rate of 88.1% . Leaf genomic DNA from T1 plantlet of different genotypes and treatments were analyzed by PCR and 16 positive lines were (obtained) (among) 31 tested lines. Anti-TrxS gene were also transformed into 1 206 (florets) of Yumai 18、Yumai 49、Yumai 70、Zhengxin 991 and 95519 by means of ovary injection. 89 seeds (7.4% of the (flowers)) and 41 plantlets (46.1% of seeds) were achieved. Leaf genomic DNA of Zhengxin 991 T1 plantlets line (the sample was mixture of 12 plantlets leaf genomic DNA) was identified as positive strain by PCR and electrophoresis analysis.更多还原