【摘要】 酶活性的变化与育性有着密切的关系。对与线粒体有关的过氧化物酶、超氧化物歧化酶、细胞色素氧化酶和琥珀酸脱氢酶进行了酶活性分析。结果表明:在花粉发育的各个时期晋A不育系的酶活性比其保持系明显要低。这表明了不育系与其保持系之间体内代谢强度的差异,也说明酶活性的变化与雄性不育现象有着直接的关系。更多还原

【Abstract】 Cytoplasmic male sterile line Jin A was derived from trispecific hybrids [（G. hirsutum）×（G.thurberi Tod D₁）]×[（G. arboreum）×（G. hirsutum）]. This study deals with Jin A male sterile and its maintainer line in activity of several enzymes.Cotyledon, leaf, pollen and anther in two developed stages （sporogenous cell division and meiosis progressed） of cytoplasmic male sterile line Jin A and its maintainer line were collected from field. In the two developed stages based on observations under micrscope, activity of peroxide （POD） , cytochrome oxidase （COD）, superoxide dismutase （SOD） and succinic dehydrogenase were determined.A correlation between CMS and varies of activity of enzyme were studied. The activity of four enzymes with mitochondrial function in cotyledon, leaf and anther of Jin A cytoplasmic male sterile and in those of its maintainer line were also studied. The results showed that activity of peroxide （POD）, cytochrome oxidase （COD）, superoxide dismutase （SOD） and succinic dehydrogenase in the anther of the sterile line were lower than those in the fertile line; but such differences were not found between the leaves of the sterile line and the fertile line, and only the activity of succinic dehydrogenase was lower in the cotyledon of the sterile line than that of fertile line. These indicated different metabolize between the sterile line and fertile lines. Peroxide （POD）, cytochrome oxidase （COD）, superoxide dismutase （SOD） and succinic dehydrogenase were important enzymes in breathing chain. These indicated difference in mitochongdrial structure and function between the sterile line and fertile lines too.更多还原