【摘要】 目的　研究人巨细胞病毒HCMV UL14 0基因在临床低传代分离株中的多态性及其与致病性的关系。方法　对 4 0株HCMV临床低传代分离株进行UL14 0基因全序列的PCR扩增 ,对 12株进行了测序及结果分析。结果　 4 0株HCMV UL14 0基因PCR扩增均阳性。测序的 12株HCMV UL14 0开放阅读框架 (ORF)均在Toledo株UL14 0 ORF的第 174位核苷酸处 ,插入一个胞嘧啶核苷酸C ,造成移码突变。与Toledo株相比 ,临床分离株新增了ScAMP磷酸化SPS和酪蛋白激酶Ⅱ磷酸化CKP两种重要功能位点 ,且其所位于的氨基酸位点可能是UL14 0蛋白的蛋白质作用位点。结论　临床分离株UL14 0基因的ORF较Toledo株多出 2 31个核苷酸 ,故两者的核苷酸及其编码产物氨基酸序列明显不同。临床分离株存在SPS及CKP两种新的重要功能位点 ,可能在HCMV UL14 0编码蛋白的生物学功能方面起重要作用。更多还原

【Abstract】 Objective To investigate the polymorphism of human cytomegalovirus UL140 gene in low passage clinical isolates and try to find the relationship between the polymorphism and different pathogeneses of congenital HCMV infection.Methods PCR was performed to amplify the entire HCMV-UL140 genes region of 40 clinical isolates. The 12 PCR products of UL140 genes were sequenced directly and the sequence data were analyzed.Results UL140 genes in 40 clinical isolates were amplified successfully. In comparison with that of Toledo, all UL140 ORF in 12 clinical isolates had an insertion at nucleotide position 174. There were two new sites in clinical isolates, SulfationcAMP phosphorylation site (SPS) and Casein kinase phosphorylation Ⅱ site (CKP), which did not exist in Toledo strain; and SPS and CKP lied in amino acid positions 80～90 and 130～150 which were the possible protein action sites.Conclusion HCMV UL140 gene in clinical isolates was 231bp longer than that of Toledo, so both nucleotide and amino acid sequences of HCMV-UL140 in clinical isolates were apparently different from that of Toledo.The functional motifs SPS and CKP in clinical isolates might have an important role in biological function of UL140 encoded protein.更多还原