【摘要】 对来自中国不同大豆产区的10个大豆胞囊线虫(Heteroderaglycines)群体及1个美国的群体rDNA中的ITS区进行了PCR扩增,相关的产物通过五种限制性内切酶进行了酶切和序列比对分析。结果表明,各群体经PCR扩增均得到一条大约1000bp大小的片段;各群体的rDNA中的ITS区用AluⅠ、CfoⅠ、MvaⅠ、RsaⅠ酶切后具有相同的酶切表型,只有AvaⅠ在来自山西太谷的群体中产生两种表型;序列分析表明,在大豆胞囊线虫种内群体之间ITS区中,ITS1和编码5.8SRNA基因区的序列完全相同,而在ITS2区有3个碱基的差异,显示了大豆胞囊线虫rDNA中的ITS区在种内群体的稳定性。更多还原

【Abstract】 The rDNA-ITS region of ten soybean cyst nematode (SCN), Heterodera glycines, populations from different soybean production areas of China and one population from USA were amplified. All populations gets the same length fragment, i.e. approximately 1000bp. The fragments were digested with five restriction enzymes, AluⅠ,CfoⅠ,MvaⅠ,RsaⅠ and AvaⅠ, of which, AluⅠ,CfoI, MvaI and RsaⅠ, produced restriction profiles identical among all populations, while AvaⅠyielded two phenotypes in the population from Taigu, Shanxi. The sequence alignment showed that the ITS1 region and 5.8S RNA gene are all identical among all the populations, while only three base pare differences were found in ITS2 region. The results indicated that rDNA-ITS region is relatively stable among the intrapopulations of Heterodera glycines.更多还原