【摘要】 生长激素 (growthhormone)是一种调节动物生长的重要激素 ,具有广泛的生理功能。本研究以鲁北白山羊、引进波尔山羊、纯繁波尔山羊、鲁北白山羊×波尔山羊的杂交 1代、回交 1代 5个不同山羊种群共 2 76个个体为研究材料 ,对山羊生长激素基因的第一内含子到第二内含子进行PCR -RFLP分析 ,扩增产物经限制性内切酶HaeⅢ、HhaⅠ、TaqⅠ等 10种内切酶酶切发现 :HaeⅢ在 5个种群中均具有多态性 ,并表现为两种基因型 (AA和AB) ,而其它的内切酶均未发现多态性位点更多还原

【Abstract】 Growth hormone is an important hormone to adjust animal growth.It has extensive physiology function. The polymorphism of goat GH gene in 276 goats including LuBei white goat,introduced boer goat, purebred breed boer goat, hybrid generation 1 of LuBei white goat and boer goat,generation of hybrid generation 1 and Boer goat were detected by PCR-RFLPs and the PCR products were digested by 10 restriction endonucleases such as HaeⅢ、HhaⅠ、TaqⅠ etc. The result showed that the HaeⅢ site had polymorphism in the 5 breeds and presented as AA and AB genotype, but othes had no polymorphims.更多还原