【摘要】 目的 :建立人肺腺癌A5 4 9 CARP耐药细胞系。方法 :采用卡铂浓度梯度递增 ,持续性作用的体外诱导法 ,MTT检测细胞耐药性。结果 :A5 4 9 CARP对卡铂耐药 ,其生物学特性和倍增时间与A5 4 9无差异。结论 :成功构建了A5 4 9 CARP耐药细胞系 ,为后续研究提供了耐药模型。更多还原

【Abstract】 Objective: To establish resistant subline A549/CARP. Methods: CARP-resistant A549(A549/CARP) was developed by different doses and the sensitivity of anticancer drugs were screened in the A549/CARP by MTT method. Result: A549/CARP was resistant to the carp drug. There was no significant difference in biologic characteristic and duplication time of A549 and A549/CARP. Conclusion: The establishment of resistant subline A549/CARP could be regarded as a model for future seeking and screening anticancer drugs.更多还原