【摘要】 目的　探讨荧光定量 PCR( fluorescence quantitative polymerase chain reaction,FQ-PCR)定量检测血清HBV-DNA的临床应用价值。方法　采用 FQ-PCR和 ELISA法检测 1 76例乙型肝炎患者血清 HBV DNA含量和 HBV血清学标志物 ,并进行比较分析。结果　 HBs Ag、HBe Ag和抗 -HBc三项阳性 ,HBs Ag、抗 -HBe、抗 -HBc三项阳性 ,HBs Ag、HBe Ag两项阳性 ,HBs Ag、抗 -HBc阳性的样品中 ,HBV DNA阳性率分别为 95 .1 %、83 .3 %、1 0 0 .0 %、81 .8% ,DNA拷贝数/ml分别为 2 .2 9× 1 0 7、1 .2 3× 1 0 6 、8.5 1× 1 0 6 、6.61× 1 0 5,其中以抗 -HBc-Ig M阳性组 DNA拷贝数为最高。结论　 FQ-PCR法检测 HBV DNA具有灵敏度高、结果可靠的优点 ,可检测 HBV感染和复制状态 ,对于乙肝的早期诊断、传染性判断和疗效考核具有重要的指导意义。更多还原

【Abstract】 Objective To explore the clinical value of FQ-PCR in detecting HBV DNA of hepatitis B patients.Methods FQ-PCR and ELISA were used to measure HBV DNA and serum markers,and the two results were analyzed.Results In 41 HBsAg(+)/HBeAg(+)/HBcAb(+) samples, 95.1% was positive by FQ-PCR,with 2.29×10 7/ml HBV DNA on average.In 60 HBsAg(+)/HBeAb(+)/HBcAb(+) samples,the average was 1.23×10 6/ml with the positive rate of 83.3%.In HBsAg(+)/HBeAg(+) samples,the average was 8.51×10 6/ml, the positive rate was 100.0%.In HBsAg(+)/HBcAb(+) samples, the results were 6.61×10 5/ml and 81.8%.Conclusion The method of FQ-PCR in detecting HBV DNA is sensitive and reliable, and can be used to monitor the true state of HBV infection. FQ-PCR has the important clinical value on early diagnosis,infectious ability judgement,curative effect assessment of hepatitis B patients.更多还原