【摘要】 目的研究局部热化疗对鼠胶质瘤PCNA和VEGF蛋白表达的影响,探讨其作为胶质瘤辅助治疗的可行性。方法将传代培养的C6细胞接种于大鼠背部皮下,肿瘤生长至1.5～2cm直径时,分组进行局部热疗、化疗、热化疗。测量肿瘤体积和重量;用免疫组化S-P法检测PCNA、VEGF蛋白表达,光镜观察肿瘤增殖状况。结果热疗、化疗、热化疗与对照组比较:瘤体缩小、瘤重减轻,PCNA和VEGF蛋白表达减低(P<0.01),且热化疗较单纯热疗和化疗减低更明显(P<0.01)。结论①热疗、化疗、热化疗能抑制肿瘤生长,且热化疗有协同作用;②热化疗一方面抑制PCNA合成与表达,抑制肿瘤增殖;另一方面抑制VEGF合成与分泌,抑制肿瘤血管再生,达到抗瘤作用。更多还原

【Abstract】 Objectives To study the effects of local thermochemotherapy on the expression of PCNA and VEGF protein of gliomas in rat, and exploxe the practicability as adjuvant therapy for treating gliomas.Methods C6 glioma cells via transmitted generation culture were subcutaneously injected to the back of rats,who were randomly divided into local heat therapy,chemotherapy,thermochemotherapy and control groups.The volume and weight of the tumors were measured.The expression of PCNA and VEGF protein were detected by S P(streptavidin peroxidase conjugated methods)immunohistochemical staining.The proliferative status of gliomas were observed by optical microscopic technique.Results Compared with the control group,the tumor′s volume,sweight and expression of PCNA and VEGF protein were decreased(P< 0 01),and thermochemotherapy group was lower than that simple hyperthermia and chemotherapy group obviously(P< 0 01).Conclusion ①Hyperthermia,chemotherapy and thermochemotherapy can inhibit the tumor growth and the thermochemotherapy has synergstic effects in the rats.②The thermochemotherapy can inhibit synthesis and expression of PCNA and inhibit the tumor′s proliferation.On the other hand,it can inhibit synthesis and secretion of VEGF,and inhibit the regeneration of tumor vessels.So,it can reach to the effect of anti tumors.更多还原